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» Bioexplorer Toolbar 
A bioinformatics toolbar designed for IE and Mozilla browsers. It provides direct access to NCBI databases (PubMed, Protein, Nucleotide, and Taxonomy) and to other biological Web resources, including search engines.
http://www.bioexplorer.net/toolbar/
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» NEBcutter
On-line DNA sequence tool to find large, non-overlapping, open reading frames and sites for all restriction enzymes-from New England BioLabs.
http://tools.neb.com/NEBcutter/index.php3
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» REBASE
The restriction enzyme database, from New England Biolabs.
http://rebase.neb.com/rebase/rebase.html
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» RestrictionMapper
RestrictionMapper is an on line restriction mapping tool. It reports exact cut points and supports circular DNA. It also includes a virtual digest function and a dilution calculator.
http://www.restrictionmapper.org
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» WatCut
Watcut is an on-line tool that offers three functions: 1. restriction mapping, 2. silent mutation scanning, and 3. scanning for amplification-created restriction sites that facilitate detection of SNP by PCR.
http://watcut.uwaterloo.ca/watcut/watcut/template.php
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» Web Primer
Design of PCR or sequencing primers.
http://seq.yeastgenome.org/cgi-bin/SGD/web-primer
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» Webcutter 2.0
Free, online software for restriction mapping nucleotide sequences.
http://users.unimi.it/~camelot/tools/cut2.html
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